Methane and ammonia emissions from a beef feedlot in western Canada for a twelve-day period in the fall. Can. J. Anim. Sci. 88: 641649. Commercial feedlot operations are becoming a mainstay in the Canadian beef industry. These large operations that typically raise thousands of animals at a time represent a large localized source of methane (CH4) and of atmospheric pollutants such as ammonia (NH3) and particulate matter. An inverse dispersion model was utilized to calculate CH4 and NH3 emissions from a commercial cattle feedlot and an adjacent runoff retention pond. The feedlot measurements were collected within the interior of the feedlot enabling a near continuous emissions record over the 12 d of the study period. Average daily emission estimates of CH4 and NH3 were 323 and 318 g animal -1d-1, respectively. The CH4 emissions represent 4% of the gross energy intake (GEI) and NH3 emissions represent 72% of the total N intake. Emissions from the runoff retention pond associated directly with the feedlot operation were approximately 2.7 and 2% of the daily average feedlot emissions of CH4 and NH3, respectively.