The atmospheric CO2 concentration is increasing, due primarily to fossil-fuel combustion and deforestation. Sequestering atmospheric C in agricultural soils is being advocated as a possibility to partially offset fossil-fuel emissions. Sequestering C in agriculture requires a change in management practices, i.e. efficient use of pesticides, irrigation, and farm machinery. The C emissions associated with a change in practices have not traditionally been incorporated comprehensively into C sequestration analyses. A full C cycle analysis has been completed for agricultural inputs, resulting in estimates of net C flux for three crop types across three tillage intensities. The full C cycle analysis includes estimates of energy use and C emissions for primary fuels, electricity, fertilizers, lime, pesticides, irrigation, seed production, and farm machinery. Total C emissions values were used in conjunction with C sequestration estimates to model net C flux to the atmosphere over time. Based on US average crop inputs, no-till emitted less CO2 from agricultural operations than did conventional tillage, with 137 and 168 kg C ha(-1) per year, respectively. Changing from conventional tillage to no-till is therefore estimated to both enhance C sequestration and decrease CO2 emissions. While the enhanced C sequestration will continue for a finite time, the reduction in net CO2 flux to the atmosphere, caused by the reduced fossil-fuel use, can continue indefinitely, as long as the alternative practice is continued. Estimates of net C flux, which are based on US average inputs, will vary across crop type and different climate regimes. The C coefficients calculated for agricultural inputs can be used to estimate C emissions and net C flux on a site-specific basis. Published by Elsevier Science B.V.