Land use change can lead to changes in a range of soil properties, including soil carbon (C) and nitrogen (N) content, bulk density and pH. Previous investigations on the effects of land use change have been biased towards the impact of forest clearing and afforestation in tropical environments. Therefore, the aim of this study is to determine the impact of a conversion from cropland to grassland on soil properties in two districts of the Lahn-Dill Highlands, Germany. We determined a land use history for the period 1945-2004 from aerial photographs and field surveys. This land use history was used to build a chronosequence of grassland sites with a different age since the conversion from cropland for both districts. Each chronosequence was sampled to determine bulk density, pH, coarse material fraction, C/N ratio and soil C and N content as a function of grassland age. Results showed that there was no clear dependency of soil properties on grassland age for both districts. It was concluded that observed differences within each district are much more related to differences in soil parent material or slope position, instead of land use. Interestingly, the reduction of the chronosequence analysis to a paired site survey led to an opposite conclusion for the Erda district because all investigated soil properties were significantly different for continuous grassland and cropland. This indicates that care is required when interpreting results from paired site surveys, especially when the equality of initial soil conditions is not (or cannot be) tested.