Prediction of soil organic C sequestration with adoption of various conservation agricultural management approaches is needed to meet the emerging market for environmental services provided by agricultural land stewardship. The soil conditioning index (SCI) is a relatively simple model used by the USDA-Natural Resources Conservation Service to predict qualitative changes in soil organic matter. Our objective was to develop a quantitative relationship between soil organic C derived from published field studies in the southeastern USA and SCI scores predicted from matching management conditions. We found that soil organic C sequestration (at 20 +/- 5 cm depth) could be reliably related to SCI across a diversity of studies in the region using the regression slope: 1.65 Mg C ha(-1) SCI(-1) [which translated into a rate of 0.25 +/- 0.04 Mg C ha(-1) yr(-1) SCI(-1) (mean +/- standard error of 31 slope estimates)]. The calibration of soil organic C on SCI scores will allow SCI to become a quantitative tool for natural resource professionals to predict soil organic C sequestration for farmers wanting to adopt conservation practices.