Among greenhouse gases, carbon dioxide (CO 2) is one of the most significant contributors to regional and global warming as well as climatic change. A field study was conducted to (i) determine the effect of soil characteristics resulting from changes in soil management practices on CO 2 flux from the soil surface to the atmosphere in transitional land from perennial forages to annual crops, and (ii) develop empirical relationships that predict CO 2 flux from soil temperature and soil water content. The CO 2 flux, soil temperature ( Ts), volumetric soil water content (theta v) were measured every 1-2 weeks in no-till (NT) and conventional till (CT) malt barley and undisturbed soil grass-alfalfa (UGA) systems in a Lihen sandy loam soil (sandy, mixed, frigid Entic Haplustoll) under irrigated and non-irrigated conditions in western North Dakota. Soil air-filled porosity (epsilon) was calculated from total soil porosity and theta v measurements. Significant differences in CO 2 fluxes between land management practices (irrigation and tillage) were observed on some measurement dates. Higher CO 2 fluxes were detected in CT plots than in NT and UGA treatments immediately after rainfall or irrigation. Soil CO 2 fluxes increased with increasing soil moisture ( R2=0.15, P<0.01) while an exponential relationship was found between CO 2 emission and Ts ( R2=0.59). Using a stepwise regression analysis procedure, a significant multiple regression equation was developed between CO 2 flux and theta v, Ts (CO 2 flux=e -3.477+0.123T s+6.381theta v ; R2=0.68, P?0.01). Not surprisingly, soil temperature was a driving factor in the equation, which accounted for approximately 59% in variation of CO 2 flux. It was concluded that less intensive tillage, such as no-till or strip tillage, along with careful irrigation management will reduce soil CO 2 evolution from land being converted from perennial forages to annual crops.