Tillage has been reported to induce changes in soil organic matter (SOM) concentrations and quality. Conversion of plow-tillage to minimum till and no-till (NT) farming enhances the SOM pool. Enrichment of the SOM pool is essential for maintaining fertility of Chernozems, advancing food security, and improving the environment. The main objective of this study was to examine the effect of different tillage systems on the SOM concentration, its quality and dynamics including CO 2 assimilation by heterotrophic bacteria and humus characteristics - the carbon (C) concentration in humic substances and the labile soil organic C fraction (SOCL) extracted with 0.1 N NaOH - as well as the molecular masses, spectroscopic parameters and physiological effects of humic acids on germinating pea ( Pisum sativum L.) seeds. Our study was conducted on a long-term experimental site on a Haplick Chernozem in the Poltava region of Ukraine over a 10-yr period from 1996 to 2006. Results indicate that conversion from conventional to reduced soil tillage systems increased SOM concentrations in 0- to 10-cm soil layer and led to the accumulation of C in fulvic acids and humins. No significant differences in SOM storage in the 0- to 100-cm layer were observed among tillage systems. However, reduced tillage systems had a higher proportion of SOCL, a lower ratio of C in humic acids/C in fulvic acids and more humic acids with molecular masses from 110 to 2000 kDa. Our study demonstrated that the quality and dynamics of SOM are closely related to soil tillage practices.
