Little is known about the crop influence and the long-term effects of tillage systems on physical and morphological properties of Vertisols in the high plateau of the intertropical zone of Mexico. The purpose of the present study was to evaluate the impact of crop rotation on the development of structure and pedofeatures of Eutric Vertisols of Guanajuato, Mexico. Disturbed and undisturbed samples from the top soil (0-20 cm) of five agroecosystems differing in crop rotation (semipermanent alfalfa, two cereal crops per year, one vegetable and one cereal crop per year, three vegetable crops per year, and dryland maize) were collected. A second no-till soil sample was obtained from a nearby site under secondary vegetation and used as reference soil. Continuous and intensive cultivation lead to a significant reduction in Vertisol organic matter and total nitrogen contents. Tillage and crop rotations affected air-dried aggregate bulk densities, soil microstructure and development of pedofeatures. Calcite crystalline pedofeatures seemed to be more directly affected by the management systems used. Nodules, the most common crystalline pedofeatures in top soil of all cultivated Vertisols, were absent in no-till soils.