The COSMOS-Flux project aimed at studying two situations that have important environmental impacts at a larger scale : the conversion tillage no tillage where different tillage systems have been applied for 14 years at the start of experiment; the conversion grassland annual crop where the introduction of temporary grassland into rotations is studied. The characterization of upper layers of soil for C and N pools, mineralization, immobilization and nitrification of N, along with characteristics of the nitrifying and denitrifying bacterial communities (activity, size and structure) were followed during 18 to 36 months after conversion. We observed that the tillage of soils untilled for 14 years, or the ploughing of the 5-year old grassland were major disturbances for the soils, which led to a very fast evolution of soil organic matter pools, N fluxes and microbial activities towards the characteristics observed for tilled and arable situations. Conversely, the shifts from till to no-till, and the establishment of grassland on soil previously cropped with annual species did not change significantly their soil characteristics at the time scale of the study. Among soil environmental variables, soil organic carbon appeared as a key driver of the observed responses.
