Nitrogen uptake pattern by cotton (Gossypium hirsutum L.) at different growth stages in response to long-term application of poultry litter (PL) in a no-till system (NT) was studied on a silt loam soil in 2009. The study was done in plots that were established in 1996 at TVREC, Belle Mina, AL, USA. Treatments included were three tillage [conventional tillage (CT), mulch-tillage (MT), and no-tillage (NT)] two cropping systems tot ton-lye (C-R; cotton in summer & cereal rye cover crop in winter), and cotton-fallow (C-F; cotton in summer & fallow in winter)] and two sources of nitrogen [PL at 100 and 200 kg N ha(-1) and ammonium nitrate (AN) at 100 kg N ha(-1)] Out of all treatment combinations only 11 important treatments were selected and arranged in a randomized complete block design and replicated 4 times. Results in 2009, showed that NT system can supply equal quantity of nitrogen compared to CT at all growth stages. No-tillage recorded similar growth, yield and total nitrogen uptake compared to CT. Application of PL at 100 kg N ha(-1) showed significantly superior plant growth compared to AN at early growth stage, but the differences disappeared as the plant growth progressed. Similar yields and nitrogen uptake were observed with application of either PL or AN at 100 kg N ha(-1). Application of a double rate of PL (200 kg N ha(-1)) resulted in significantly higher nitrogen uptake compared to that of PL or AN at 100 kg N ha(-1), but a significant yield advantage was not observed with this higher rate. Of the total nitrogen extracted by cotton at maturity, 50% uptake was completed by early flowering stage and 97% was completed by boll development stage. At maturity, the majority of nitrogen (52%) was partitioned into seeds, while the rest was distributed into leaves (16%), stems (18%) and reproductive parts (14%). winter rye cover crop did not influence nitrogen uptake. (C) 2012 Friends Science Publishers