A study was conducted to evaluate the influence of tillage systems on the incidence of Tilletia indica (Karnal bunt) in a rice-wheat system that is the most popular and prevalent crop rotation in the Indo-Gangetic Plains of India. A total of 906 samples, were drawn from the farmers' fields during the month of April during the 3 years study period. The samples collected were 365, 171, and 370 from the zero tillage (ZT), furrow irrigated raise bed system (FIRBS), and conventional till (CT) sown fields, respectively. The disease incidence, incidence index and percent-infected samples were calculated and statistically analyzed. Results showed that ZT fields had the lowest mean incidence of Karnal bunt, i.e. 9.00% infected samples in comparison to 18.10% and 16.20% under FIRBS and CT, respectively. Similarly, the average infection in infected samples was equal in FIRBS and CT, but the samples from ZT were statistically lower. KB incidence index showed a similar trend. ZT has shown a reduced incidence of KB in comparison to the raised bed (FIRBS) and CT systems. If ZT is followed for a period of a few years, it may help in reducing the effective soil inoculum and reducing the disease incidence over time.