Conservation management systems can improve soil organic matter stocks and contribute to atmospheric C mitigation. This study was carried out in a 18-year long-term experiment conducted on a subtropical Acrisol in Southern Brazil to assess the potential of tillage systems [conventional tillage (CT) and no-till (NT)], cropping systems [oat/maize (O/M), vetch/maize (V/M) and oat+vetch/maize+cowpea (OV/MC)] and N fertilization [0 kg N ha -1 year -1 (0 N) and 180 kg N ha -1 year -1 (180 N)] for mitigating atmospheric C. For that, the soil organic carbon (SOC) accumulation and the C equivalent (CE) costs of the investigated management systems were taken into account in comparison to the CT O/M 0 N used as reference system. No-till is known to produce a less oxidative environment than CT and resulted in SOC accumulation, mainly in the 0-5 cm soil layer, at rates related to the addition of crop residues, which were increased by legume cover crops and N fertilization. Considering the reference treatment, the SOC accumulation rates in the 0-20 cm layer varied from 0.09 to 0.34 Mg ha -1 year -1 in CT and from 0.19 to 0.65 Mg ha -1 year-1 in NT. However, the SOC accumulation rates peaked during the first years (5th to 9th) after the adoption of the management practices and decreased exponentially over time, indicating that conservation soil management was a short-term strategy for atmospheric C mitigation. On the other hand, when the CE costs of tillage operations were taken into account, the benefits of NT to C mitigation compared to CT were enhanced. When CE costs related to N-based fertilizers were taken into account, the increases in SOC accumulation due to N did not necessarily improve atmospheric C mitigation, although this does not diminish the agricultural and economic importance of inorganic N fertilization.