Winter cover crops are not only one of effective agricultural management practices to control weeds but also can improve soil fertility, resulting in increasing crop productions. Up to now, however, little is known about information on how much of soil soluble organic carbon (C) incorporates into the soils applied with winter cover crops, which is a prerequisite to design strategies that improve C sequestration in agricultural ecosystems. The aims of this study were to: (1) assess the effects of winter cover crops on soluble organic carbon (SOC) pools using different extraction methods (KCl extractable organic C; microbial biomass) and microbial community functional diversity, and (2) quantify how much of the potentially mineralizable organic C pools (C 0) incorporates into the soils and associated half-life of SOC remaining under seven cover crops and nil-crop control (CK) in temperate agricultural soils of southern Australia. Cover crop treatments are cereal rye, wheat, saia oats, vetch, field peas, mustard and the mixture of cereal rye and vetch. Results showed that the CK treatment had higher soil moisture content and lower soluble organic nitrogen (SON) compared to the cover crop treatments. Among the cover crop treatments, there was significantly higher SON in the wheat, oats and vetch treatments than in the other treatments. The oats treatment had the highest amount of cumulative CO 2-C than any other treatments over one-month incubation experiment. An exponential regression approach for C mineralization was used to estimate C o and soil samples under the cover crops can be divided into four groups depending on C o. The principal component analysis of the MicroResp TM profiles showed that the CK treatment was significantly different from the cover crop treatments. The cover crop treatments with wheat, vetch and peas as well as mustard form a cluster which was significantly different from the other clusters. In addition, the vetch, field peas and mustard treatments showed higher Shannon diversity H and Evenness (E) and Simpson diversity H compared to the other cover crop treatments with the lowest Shannon H and E at CK. In conclusion, overall, the vetch and field peas as well as wheat winter cover crop may be better management practices for agricultural ecosystems in southern Australia.
