Citation Information

  • Title : Immunoassay based on monoclonal antibodies versus LC-MS: deoxynivalenol in wheat and flour in Southern Brazil.
  • Source : Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
  • Publisher : Taylor & Francis
  • Volume : 28
  • Issue : 8
  • Pages : 1083-1090
  • Year : 2011
  • DOI : 10.1080/19440049
  • ISBN : 10.1080/19440049
  • Document Type : Journal Article
  • Language : English
  • Authors:
    • Kawamura, O.
    • Mallmann, C.
    • Itano, E.
    • Ono, E.
    • Takabayashi, C.
    • Santos, J.
    • Hirooka, E.
  • Climates: Humid subtropical (Cwa, Cfa). Temperate (C).
  • Cropping Systems: Wheat.
  • Countries: Brazil.

Summary

An indirect competitive enzyme-linked immunosorbent assay (ELISA) method using a monoclonal antibody for deoxynivalenol (DON) detection in wheat and flour was standardised and validated (detection limit - 177.1 g kg -1) and its performance was compared with LC-MS, (quantification limit=140 g kg -1). DON recovery ranged from 88.7% to 122.6% for wheat grain and from 70.6% to 139.3% for flour. Among the 38 wheat samples evaluated, DON was detected in 29 samples (76.3%) by ic-ELISA (281.6-12 291.4 g kg -1) and in 22 samples (57.9%) by LC-MS (155.3-9906.9 g kg -1). The 0.93 correlation coefficient between ic-ELISA and LC-MS data in 19 positive DON wheat samples demonstrated the reliability and efficiency of ic-ELISA. Results indicated that standardised ic-ELISA was suitable for DON screening in wheat samples and the need for continuous monitoring of mycotoxin levels in foodstuffs.

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