Management practices, such as no tillage (NT) and intensive cropping, have potential to increase C and N sequestration in agricultural soils. The objectives of this study were to investigate the impacts of conventional tillage (CT), NT, and cropping intensity on soil organic C (SOC) and N (SON) sequestration and on distribution within aggregate-size fractions in a central Texas soil after 20 years of treatment imposition. Tillage regime and cropping sequence significantly impacted both SOC and SON sequestration. At 0-5 cm, NT increased SOC storage compared to CT by 33% and 97% and SON storage by 25% and 117% for a sorghum/wheat/soybean (SWS) rotation and a continuous sorghum monoculture, respectively. Total SOC and SON storage at both 0-5 and 5-15 cm was greater for SWS than continuous sorghum regardless of tillage regime. The majority of SOC and SON storage at 0-5 cm was observed in 250-m to 2-mm aggregates, and at 5-15 cm, in the >2-mm and 250-m to 2-mm fractions. Averaged across cropping sequences at 0-5 cm, NT increased SOC storage compared to CT by 212%, 96%, 0%, and 31%, and SON storage by 122%, 92%, 0%, and 37% in >2-mm, 250-m to 2-mm, 53- to 250-m, and