Barley is a cool-weather cereal grain primarily produced on dryland farms in Sistan region. In order to study the effect of different proportions of manure and chemical fertilizer and water stress on grain yield and grain nutrient content in barley an experiment was conducted as split plot randomized complete block design with three replications in research field of Zabol University, 2009. Water stress treatments consisted of: water stress in grain filling stage (S 1) and control (S 2) as the main factor and different proportions of manure and chemical fertilizer treatment consisted of: 100% manure (N 1), 100% chemical fertilizer (N 2), 50% manure+50% chemical fertilizer (N 3), 75% manure+25% chemical fertilizer (N 4) and control (N 5) as sub factor in this experiment. Results illustrated that the effect of drought stress in grain filling stage treatment on all grain yield and yield components with the exception of ear weight, were significant. Drought stress in grain filling stage strongly decreased grain yield but its effect was not very strong on another traits. With the exception of grain number/ear and ear weight, fertilizer treatments had significant effect on grain yields and yield components. Grain nutrient content not affected by water stress but among different proportions of fertilizer treatments, 100% manure (N 1) caused to increase of these elements in grain.
