Two experiments were established in 1969 and 1970 near Sidney, NE, to determine the effect of moldboard plow (plow), sub-tillage (sub-till), and no-tillage (no-till) fallow management on soil properties, biological activities, and carbon and nitrogen cycling. One experiment was on land which had been broken from sod in 1920, seeded to crested wheatgrass [Agropyron cristatum (L.) Gaertn.] from 1957 to 1967, and cultivated for wheat again in 1967 (Previously Cultivated site). The second experiment was established on land that was in native mixed prairie sod until 1969 (Native Sod site), and compared the three tillage management practices listed above in a winter wheat-fallow system as well as replicated plots remaining in sod. Soil sampling done 10-12 years after these experiments were initiated, indicated that the biological environment near the soil surface (0-30 cm) with no-till was often cooler and wetter than that with conventional tillage management practices, especially moldboard plowing. Biological activity and organic C and N reserves were concentrated nearer the soil surface (0-7.6 cm) with no-tillage, resulting in greater potential for tie-up of plant available N in organic forms. However, regardless of tillage practice with wheat-fallow management at either site, long-term (22-27 years) losses of soil organic C from surface soil (0-30 cm) ranged from 12 to 32% (320-530 kg C ha(-1) year(-1)), respectively, for no-till and plowing. These soil C losses were closely approximated by losses measured to a depth of 122 cm, indicating that under the cropping, tillage, and climatic conditions of this study, soil C changes were adequately monitored by sampling to a depth of 30 cm within which most C loss occurs. No-till management maintains a protective surface cover of residue and partially decomposed materials near the soil surface. However, the decline in soil organic matter, and associated degradation in soil quality, will likely only be slowed by increasing C inputs to soil through use of a more intensive cropping system which increases the time of cropping and reduces the time in fallow. (C) 1998 Elsevier Science B.V. All rights reserved.