This study uses turbulence, net radiation and soil data from International Energy Balance Experiment (EBEX-2000) to study the characteristics of sensible and latent heat flux under thermal internal boundary layer which is induced by heterogeneous irrigation. Energy balance closure on irrigated days was compared with that on non-irrigated days. Schotanus correction and Webb correction were applied when calculating turbulence heat fluxes and their influences were also analyzed. During this research, eddy covariance method is used. Results indicate that turbulence heat fluxes in the surface layer are affected by thermal internal boundary layer resulted from heterogeneous irrigation, this interaction leads to a decrease of sensible heat flux and a oscillation of latent heat flux. This phenomenon is more significant at 8.7 m than that at 2.7 m. The existence of thermal internal boundary layer induced by heterogeneous irrigation result in a decreased energy balance ratio of 0.65; while with no internal thermal boundary layer, the energy balance ratio is close to 0.70. Schotanus correction results in a significant diminution of sensible heat flux in this experiment. The daily average of Schotanus correction reach up to -8 W/m(2), almost 4% of the net radiation. The daily average of Webb correction to latent heat flux is about 2 W/m(2), having slight influence on energy balance closure.