The aim of the study was to assess the dry matter, starch, vitamin C, glycoalkaloids and nitrates content in potato tubers from organic system cultivated in light, sandy soil of IHAR Jadwisin (Mazovia). The objective of this work was to evaluate the tuber chemical composition of 8 cultivars of potatoes under the influence of the irrigation. Irrigation had no effect on dry matter, starch and vitamin C, but significantly affected glycoalkaloids and nitrates. The study showed a significant effect of variety and years of research on the content of dry matter, starch, vitamin C, nitrates and glicoalkaloids. Genotypic differences were the most evident in the case of nitrates and glycoalkaloids content. Under the influence of irrigation decreased the nitrate content and increased content of glycoalkaloids.