Given the political targets, it can be expected that in Europe, energy production from agricultural land will increase and that improved systems for its production are needed. Therefore, a four year field trial was conducted on one site in south-western Germany to compare and evaluate the biomass and energy yield performance of important energy crops. Six energy cropping systems with the potential to produce biomass for first and second-generation biofuels were selected. The systems were short rotation willow coppice, miscanthus, switchgrass, energy maize and two different crop rotation systems including winter oilseed rape, winter wheat and winter triticale. The two crop rotation systems were managed in either conventional tillage or no-till soil cultivation systems. The second test parameter was three different crop-specific nitrogen application levels. The performance of the energy cropping systems was evaluated by measuring the biomass yields and calculating the energy yields, as well as through an energy balance and nitrogen budget. Results show the superiority of the annual energy crop maize in dry matter yield (DMY) and primary net energy yield (PNEY=difference between the primary energy yield (DMY * lower heating value) and the energy consumption) performance with peak values at the highest N-application level of 19.1 t DM ha -1 a -1 and 350 GJ ha -1 a -1, respectively. The highest yielding perennial crop was miscanthus with 18.1 t ha -1 a -1 DMY and a PNEY of 277 GJ ha -1 a -1, followed by willow with 15.2 t ha -1 a -1 and 258 GJ ha -1 a -1, at the highest N-application level. Switchgrass showed the lowest yields of the perennial crops with 14.1 t ha -1 a -1 DMY at the highest N-application level. The yields of the two crop rotation systems did not differ significantly and amounted to 14.6 t ha -1 a -1 DMY of both grain and straw at the highest N-application level. Willow showed the significantly highest energy use efficiency (output (PNEY):input (energy consumption)-ratio) with 99 GJ energy output per GJ fossil energy input at the lowest N-application level (no fertilizer). The two crop rotation systems had the lowest energy use efficiency with 20 GJ GJ -1 for the production of total aboveground biomass. Energy maize gave the best energy yield performance but at a relatively high energy input, whereas willow and miscanthus as perennial energy crops combine high yields with low inputs. Results suggest that no-till systems had no negative impact on biomass and energy yields, but that there was also no positive impact on energy saving.