Tillage systems may affect soil C sequestration, with a potential impact on crop productivity or organic matter mineralization. We evaluated crop yield, C inputs to the soil, and in situ CO 2-C fluxes under no-till and conventional tillage (disc tillage) during the 3- to 6-year period from the installation of an experiment in an Entic Haplustoll of the Semiarid Pampean Region of Argentina to elucidate the mechanisms responsible for possible management-induced soil organic matter changes. Yield and biomass production were greater under no-till than disc tillage for all the crops included in the rotation (oat + hairy vetch ( Vicia villosa ssp. villosa), maize, wheat and oat). This result was attributed to the higher soil water content under no-till. Carbon inputs to the soil averaged 4 Mg C ha -1 year -1 under no-till and 3 Mg C ha -1 year -1 under disc tillage. Soil temperature was similar between tillage systems and CO 2-C emission was approximately 4 Mg C ha -1 year -1, with significant but small differences between treatments (~0.2 Mg C ha -1 year -1). Carbon balance of the soil was nearly equilibrated under no-till; meanwhile, greater C losses as CO 2 than inputs in crop residues were measured under conventional tillage. Organic C in the soil was 5.4 Mg ha -1 higher under no-till than the disc tillage treatment 6 years after initiation of the experiment. Results showed that in our semiarid environment, C sequestration occurred under no-till but not conventional tillage. The sequestration process was attributed to the effect of the tillage system on crop productivity rather than on the mineralization intensity of soil organic pools.
