Growth of agriculture and livestock can result in opening new areas for planting and has increased research to obtain sustainable production systems with lower impact and soil degradation. Soil management and cover plant alters their properties, mainly, microbiological one, leading changes on functional microorganisms density. The aim of this work was to evaluate the effect of management systems and different planting systems on density of functional groups of microorganisms in cerrado soil. Soil samples were collected in two layers (0-5cm e 5-20cm) and two periods (dry and wet season). The treatments studied were: no-tillage (NT); no-tillage with crop rotation (NTCR); conventional tillage (CT); conventional tillage with crop rotation CTCR) and native Cerrado (mesophytic forest). Native Cerrado showed higher microbial density at both layers and periods of evaluation. In general, the treatments NTCR and NT had the highest microbial density at superficial layers. The results of linear correlation between functional groups of microorganisms and chemical soil varied among tillage system. It was concluded that cerrado soil presented higher microbial density and microbial density was similar among soil management at dry period.