Ruminal fermentation, nutrient digestion, and flows to the duodenum in growing cattle fed differently produced canola protein supplements were studied in a 4 x 4 Latin square design using Speckle Park heifers (initial BW = 451 +/- 26 kg). Canola protein supplement treatments consisted of the following: 1) 8.78% regular canola meal (RCM); 2) 9.25% RCM plus 1.80% canola oil (RCMO); 3) 11.1% canola press-cake from biodiesel oil extraction (CPC); and 4) 8.14% high ruminally undegradable protein (RUP) canola meal (RUCM) plus 1.32% canola oil (RUCMO). Experimental diets also contained 39.9, 40.2, 39.9, and 39.9% barley grain; 31.7, 31.4, 31.2, and 31.4% barley silage; and 17.5, 15.2, 15.6, and 16.5% oat hulls for the RCM, RCMO, CPC, and RUCMO diets, respectively. Feeding the CPC, RCMO, and RUCMO diets decreased (P