Sulfur deficiency symptoms are more often observed in crops at early stages of growth since S can be easily leached from the surface soil. The objectives of this study were to evaluate some of the popular rotation crops grown in Brazil for tolerance to low external S levels and to determine the critical tissue concentration for S deficiency during early stages of growth. Germinated seedlings of soybean [ Glycine max (L.) Merr.], rice ( Oryza sativa L.), maize ( Zea mays L.), field bean ( Phaseolus vulgaris L.), wheat ( Triticum aestivum L.), cotton ( Gossypium spp.), sorghum ( Sorghum bicolor L.), and sunflower ( Helianthus annuus L.) were transferred to water culture with 0.0 to 32.0 mg S L -1 and were grown for 29 d. The minimum S concentration required in nutrient solutions was 2.0 mg L -1 for sunflower; 1.0 mg L -1 for cotton, sorghum, wheat, and soybean; and 0.5 mg L -1 or less for field bean, rice, and maize. All crops achieved optimum growth at 2.0 mg S L -1. Critical shoot S concentration at early stages of growth was 0.8 g kg -1 in maize and soybean; 1.1 to 1.3 g kg -1 in cotton, sorghum, and rice; and 1.4 to 1.6 g kg -1 in wheat, sunflower, and field bean. Our results demonstrate that the tolerance to low external S (