This manual presents a set of protocols for the production of doubled haploid plants in 22 major crops species including 4 tree species, and includes protocols from different germplasm of the same species. The crops covered include barley, wheat, maize, rice, triticale, rye, oats, durum wheat, timothy grass ( Phleum pratense), ryegrass ( Lolium), rape, broccoli, tobacco, potato, flax/linseed, sugarbeet, asparagus, onion, apple, poplar, cork oak ( Quercus suber), and citrus. All steps of doubled haploid production are detailed from donor plant growth conditions, through in vitro procedures, media composition and preparation, to regeneration of haploid plants and chromosome doubling methods. The practical protocols are supplemented with a list of published protocols for other crop plants, and separate chapters deal with major application of doubled haploids in breeding, mutant production, transgenesis, genetic mapping and genomics.
