The plastochron, which is the time interval between the appearance of two successive nodes and the final node number (FNN) are important variables of the vegetative development in soybean. The objective of this study was to determine the plastochron and the FNN of soybean ( Glycine max (L.) Merrill) cultivars sown in different dates under irrigated conditions in a subtropical location. A field experiment was carried out in Santa Maria, RS, Brazil during 2005/2006 growing season, with three sowing dates (09/11/2005, 09/12/2005 and 28/01/2006). It was used 15 soybean cultivars recommended for the Central and North Argentina, and South and Southeast Brazil. The variables measured were the number of nodes (NN) and the (FNN) on the main stem. The plastochron was estimated by the inverse of the slope of the linear regression between NN and the thermal time (base temperature=10degreesC) accumulated since plant emergence. The late sowing resulted in lower plastochron and FNN, probably because of the lower photoperiod under which the plants were submitted in the node phase, a typical response of short day plants.