The role of plant growth regulators (PGR) in nitrogen (N) fertilization of spring wheat and oats (CCC [chlormequat]), fodder barley (etephon/mepiquat) an oilseed rape (etephone) in crop rotation was studied in 1993-96 on loamy clay soil. Carry over effect of the N fertilization rates (0-180 kg/ha) was evaluated in 1997. N fertilization rate for the best grain/seed yield (120-150 kg/ha) was not affected by PGRs. The seed and N yields of oilseed rape were improved frequently by the recommended use of PGR. The yield of oats increased in 1995-96. Even though PGR effectively shortened the plant height of spring wheat, the grain yield increased only in 1995. N yield of wheat grains was not increased. Response of fodder barley to PGR was insignificant or even negative in 1995. The data suggest that PGRs may decrease some N leaching at high N rates by improving N uptake by grain/seeds, if the yield is improved. The carryover study showed that in soils with no N fertilization, as well as in soils of high N rates, N uptake was higher than in soils with moderate N fertilization (60-90 kg/ha), independent of PGRs. According to soil mineral N contents, N leaching risk was significant (15-35 kg/ha) only after dry and warm late seasons. After a favourable season of high yields, the N rates did not significantly affect soil mineral N contents.