The central USA contains some of the most productive agricultural land of the world. Due to the high proportion of land area committed to crops and pasture in this region, the carbon (C) stored and greenhouse gas (GHG) emission due to agriculture represent a large percentage of the total for the USA. Our objective was to summarize potential soil organic C (SOC) sequestration and GHG emission from this region and identify how tillage and cropping system interact to modify these processes. Conservation tillage (CST), including no-tillage (NT), has become more widespread in the region abating erosion and loss of organic rich topsoil and sequestering SOC.