No-tillage crop production has become an accepted practice throughout the U.S. The Kyoto Protocol on climate change has prompted great interest in conservation tillage as a management strategy to help sequester CO2 from the atmosphere into soil organic matter. Numerous reports published in recent years indicate a large variation in the amount of potential soil organic carbon (SOC) storage with no tillage (NT) compared with conventional tillage (CT). Environmental controls (i.e., macroclimatic variables of temperature and precipitation) may limit the potential of NT to store SOC. We synthesized available data on SOC storage with NT compared with CT from published reports representing 111 comparisons from 39 locations in 19 states and provinces across the U.S. and Canada. These sites provided a climatic continuum of mean annual temperature and precipitation, which was used to identify potential SOC storage limitations with NT. Soil organic C storage potential under NT was greatest (~0.050 kg · m -2· yr-1) in subhumid regions of North America with mean annual precipitation-to-potential evapotranspiration ratios of 1.1 to 1.4 mm · mm-1. Although NT is important for water conservation, aggregation, and protection of the soil surface from wind and water erosion in all climates, potential SOC storage with NT compared with CT was lowest in cold and dry climates, perhaps due to prevailing cropping systems that relied on low-intensity cropping, which limited C fixation. Published data indicate that increasing cropping intensity to utilize a greater fraction of available water in cold and dry climates can increase potential SOC storage with NT. These analyses indicate greatest potential SOC storage with NT would be most likely in the relatively mild climatic regions rather than extreme environments.