The objective of this study was to verify the biomass yield potential of different cover crops in a Cerrado region, in Selviria, Mato Grosso do Sul, Brazil. The work was conducted in the Experimental University Farm of the Paulista State University (UNESP). The experimental design was the randomized complete blocks in strips with splitsplit plot: the plots were the cover crops Mucuna aterrima, Pennisetum americanum, Crotalaria juncea, Cajanus cajan and a fallow area; the split plots were the no-tillage and the conventional tillage, and the splitsplit plots were corn ( Zea mays L.), soybean ( Glycine max (L.) Merrill), and cotton ( Gossypium hirsutum L.) crops. In the winter irrigated Phaseolus vulgaris L. was the crop. In the spring/summer season the cover crop with the greatest biomass yield was Pennisetum americanum. Crotalaria juncea and the fallow area showed medium results while Mucuna aterrima and Cajanus cajan were those with the least yields. Pennisetum americanum was the crop cover with the biggest production potential of dry biomass. The cultivation systems did not influence the dry biomass of the cover crops. Only Pennisetum americanum production was influenced by crop sequence.