Fungal mycoflora and mycotoxin contamination were determined in 86 samples (21 maize, 21 wheat, 11 barley, 4 oats, 1 rye, 12 soya, 6 sunflower, 4 colza, 3 rice, 3 triticale), coming from the south-eastern part of Romania during the 2008 to 2010 period. The most frequent fungal contaminants belonged to the Aspergillus and Fusarium genera, maize was the most contaminated cereal. The main toxinogenic species identified were A. flavus, A. fumigatus, F. graminearum, F. culmorum in all cereals Aflatoxin B1 (AFB1), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEA) and fumonisins (FUMO), contents were analyzed by ELISA. More than 90% of the samples were found to be contaminated by at least one toxin. The most frequent mycotoxin was the deoxynivalenol (71.60%). Around 40% of samples were contaminated with AFB1 and FB. Ochratoxine A and zearalenone were found in 16% and 32% of samples respectively. These results demonstrated that cereals produced in Romania present a particular pattern of fungal mycoflora and mycotoxin contamination since DON, ZEA and FUMO as well as AFB1 and OTA were observed.
