Application of organic amendments to soil is an important management strategy for enhancing the restoration of degraded soils and providing better soil conditions to below-ground soil microbial composition and above-ground plant community development. This study was conducted to investigate the effect of organic amendments (poultry manure - PM; white clover residues - WCR), a mineral N fertilizer (urea N - UN), or mixtures of these fertilizers on microbial activity and nitrogen (N) mineralization through both soil analysis (laboratory incubation) and aboveground maize (Zea mays L) growth (pot experiment). In the incubation experiment, soil was amended with PM, WCR, PM + WCR, UN, UN + PM, UN + WCR, and UN + PM + WCR at the rate equivalent to 200 mg N kg(-1) soil. Pot experiment was conducted in a glasshouse using same amendments to examine the response of maize seedlings to these treatments. Organic amendments and UN applied alone or in mixtures increased soil microbial biomass compared to the control. Among N amendments, the highest evaluation of CO2-C (47.7 mg kg(-1) day(-1)), microbial biomass C (434 mg kg) and microbial biomass N (86 mg kg(-1)) were recorded in the UN + PM + WCR while the lowest values were recorded in UN. It is estimated that 9-18% of the applied N had been assimilated into microbial N pool after 105 days. Mineralization of N was higher in the fertilized soil and ranged between 85 and 192 mg N kg(-1) compared with 46 mg N kg(-1) in the control. The net cumulative N mineralized (NCNM) ranged between 43 and 169 mg kg(-1) while the net cumulative N nitrified (NCNN) ranged between 16 and 69%. Combined application of UN + PM + WCR exhibited the highest NCNM and NCNN. On average, percentage conversion of added N into NO3--N was: 21% from organic sources, 40% from UN and 52% from UN + organic sources. The apparent recovery of added N (ANR) from PM, WCR and PM + WCR was 20, 24 and 45%, respectively, while UN, UN + PM, UN + WCR and UN + PM + WCR exhibited 50, 57, 64, and 73% ANR, respectively. Results obtained from the pot experiment (on maize) were consistent with the total mineral N (TMN) released from different amendments and highly significant correlations existed between TMN and plant dry matter yield (r(2) = 0.92) and TMN and N uptake of plants (r(2) = 0.89). The present study demonstrates the existence of substantial amount of N reserve present in organic substrates, which can be transformed into inorganic N pool and can be taken into account as potential sources in the management of the nutrient poor soils and crop growth. (C) 2011 Published by Elsevier B.V.
