Soil management practices can alter the natural balance at the soil-plant-atmosphere ecosystem interface, which can significantly affect the environment. This study compared CO2 fluxes in conventional tillage (CT) and no-tillage (NT) corn (Zea mays L.) production systems receiving poultry litter (PL) and ammonium nitrate (AN) fertilizers on a Decatur silt loam soil in the Tennessee Valley region of North Alabama from Spring 2008 to Fall 2009. Soil CO2 flux in CT plots (9.5 kg CO2 ha(-1) day(-1)) was significantly greater than that in NT plots (4.9 kg CO2 ha(-1) day(-1) in summer. Soil CO2 fluxes were lowest in fall where CT plots had a mean soil CO2 emission of 0.8 kg CO2 ha(-1) day(-1), while plots under NT and grass fallow system were sinks of CO2 with fluxes -0.6 and -1.0 kg CO2 ha(-1) day(-1), respectively. Mean soil CO2 flux averaged over seasons in NT plots was 36% lower than that in CT plots. Grass fallow plots were net sinks of CO2 with a mean CO2 flux of -0.4 kg CO2 ha(-1) day(-1). Our study showed that application of PL or AN fertilizer in NT systems can significantly reduce soil CO2 emissions compared to CT systems in corn production.