Agricultural management practices play an important role in greenhouse gases (GHG) emissions due to their impact on the soil microenvironment. In this study, two experiments were performed to investigate the influence of tillage and N fertilization on GHG production at the macroaggregate scale. In the first experiment, soil macroaggregates collected from a field experiment comparing various soil management systems (CT, conventional tillage; NT, no-tillage) and N fertilization types (a control treatment without N and mineral N and organic N with pig slurry treatments both at 150 kg N ha-(1)) were incubated for 35 days. Methane (CH4), carbon dioxide (CO2) and nitrous oxide (N2O) production was quantified at regular time intervals by gas chromatography. In the second experiment, the effects of fertilization type and soil moisture on the relative importance of nitrification and denitrification processes in N2O emission from soil macroaggregates were quantified. Nitrate ammonium, macroaggregate-C concentration, macroaggregate water-stability, microbial biomass-C and N (MBC and MBN, respectively) and water-soluble C (WSC) were determined. While NT macroaggregates showed methanotrophic activity, CT macroaggregates acted as net CH4 producers. However, no significant differences were found between tillage systems on the fluxes and cumulative emissions of CO2 and N2O. Greatest cumulative CO2 emissions, macroaggregate-C concentration and WSC were found in the organic N fertilization treatment and the lowest in the control treatment. Moreover, a tillage and N fertilization interactive effect was found in macroaggregate CO2 production: while the different types of N fertilizers had no effects on the emission of CO2 in the NT macroaggregates, a greater CO2 production in the CT macroaggregates was observed for the organic fertilization treatment compared with the mineral and control treatments. The highest N2O losses' due to nitrification were found in the mineral N treatment while denitrification was the main factor affecting N2O losses in the organic N treatment. Our results suggest that agricultural management practices such as tillage and N fertilization regulate GHG production in macroaggregates through changes in the proportion of C and N substrates and in microbial activity. (C) 2013 Elsevier Ltd. All rights reserved.
