Quantitative functional relationships between temperature and fiber quality are needed to improve predictive capability of cotton ( Gossypium hirsutum L.) models. An experiment was conducted by varying day/night temperatures, 22/14, 26/18, 30/22, and 34/26°C, imposed at flowering. Upland cotton cultivar, TM-1, was seeded in the soil bins using fine sand as the rooting medium and allowed to grow under optimum water and nutrients. Flowers and bolls were tagged daily to estimate the boll maturation period. Plant height and node numbers were recorded from emergence to 21 d after treatment. Stem, leaf, boll dry weights, and boll numbers were recorded at maturity. Measured fiber quality parameters were regressed against temperature to develop mathematical functions for modeling. The optimum temperature for biomass was between 18.1 and 21.5°C and biomass declined by 10% at 25.5°C and 19% at 29.5°C. More bolls were produced at 25.5°C, but declined sharply at 29.5°C. Reproductive potential, boll mass per unit total weight, peaked at 25.5°C and was lower by 21% at 18.1°C and 53% at 29.5°C. Fiber micronaire and uniformity increased with temperature up to 26°C and declined at higher temperature, while fiber strength increased linearly with temperature. Fiber length increased linearly from 18 to 22°C, and declined at higher temperatures. Fiber micronaire was more responsive to changes in temperature followed by strength, length, and uniformity. The functional relationships between temperature and fiber properties will be useful to optimize management decisions such as planting dates and to develop fiber submodel under optimal water and nutrient conditions.