The production and consumption of the greenhouse gases (GHGs) methane (CH 4), carbon dioxide (CO 2) and nitrous oxide (N 2O) in soil profile are poorly understood. This work sought to quantify the GHG production and consumption at seven depths (0-30, 30-60, 60-90, 90-150, 150-200, 200-250 and 250-300 cm) in a long-term field experiment with a winter wheat-summer maize rotation system, and four N application rates (0; 200; 400 and 600 kg N ha -1 year -1) in the North China Plain. The gas samples were taken twice a week and analyzed by gas chromatography. GHG production and consumption in soil layers were inferred using Fick's law. Results showed nitrogen application significantly increased N 2O fluxes in soil down to 90 cm but did not affect CH 4 and CO 2 fluxes. Soil moisture played an important role in soil profile GHG fluxes; both CH 4 consumption and CO 2 fluxes in and from soil tended to decrease with increasing soil water filled pore space (WFPS). The top 0-60 cm of soil was a sink of atmospheric CH 4, and a source of both CO 2 and N 2O, more than 90% of the annual cumulative GHG fluxes originated at depths shallower than 90 cm; the subsoil (>90 cm) was not a major source or sink of GHG, rather it acted as a 'reservoir'. This study provides quantitative evidence for the production and consumption of CH 4, CO 2 and N 2O in the soil profile.
