Interest in the use of biochar to improve soil productivity has rapidly increased. Nitrogen (N) loss, retention and bioavailability in biochar-amended soils fertilized with NH4+-N and NO3--N were studied using leaching and pot experiments. NO3--N leaching from the soils fertilized with NH4+-N and NO3--N was significantly reduced by biochar addition. NH4+-N leaching from the NO3--N fertilized soil was also significantly reduced by biochar, while no significant effect was observed for the NH4+-N fertilized soil. Also lower NH4+-N and NO3--N were leached from NO3--N fertilized soil with maize (Zea mays L) grown. Mitigation of N leaching loss following biochar addition is mainly attributed to the increase in soil water holding capacity (WHC), NH4+ adsorption and enhanced N immobilization. Biochar addition stimulated maize growth, both above and below ground. Biochar also increased N utilization efficiency (NUE) of maize but decreased N accumulation efficiency (NAE), indicating that biochar addition may improve N bioavailability in agricultural soils. Therefore, reduction of N leaching, and increase of N retention and bioavailability in agricultural soils can potentially decrease the N fertilizer demand for crop growth.