The removal of corn residue for bioethanol may require changes in current tillage and fertilization practices to minimize potential alterations to the soil environment that may lead to increase in greenhouse gas (GHG) emission. The objectives of this study were to examine how tillage, N fertilization rates, residue removal, and their interactions affect CO2, and N2O soil surface emissions. Greater CO2 emission coincided with higher soil temperatures typically observed with conventional tillage (CT) compared with no-tillage (NT), resulting in greater annual cumulative CO2 emission in CT (18.1 CO2 Mg ha-1 yr-1) compared with NT (16.2 CO2 Mg ha-1 yr-1) in 2009 and 2010 across sites. However, drier soil conditions during the growing season in 2011 lead to higher soil temperatures compared with 2009 and 2010. Consequently, annual cumulative CO2 emission from NT with 50 and 100% residue removal was (19.5 CO2 Mg ha-1 yr-1) greater than that from CT (17.8 CO2 Mg ha-1 yr-1) across all residue removal rates and from NT (17.5 CO2 Mg ha-1 yr-1) with no residue removal, respectively across all N rates in the Ames central site (AC) in 2011. In the Armstrong southwest site (ASW) site, there were no significant differences between tillage or residue removal rates for annual cumulative CO2 emission (19.9 CO2 Mg ha-1 yr-1) in 2011. Although N2O emission was considerably lower than CO2 emission, differences in N fertilization rates did have a significant impact on global warming potential once these gases were converted on the basis of their radiative forcing of the atmosphere.