Tillage affects the ability of coarse-textured soils of the southeastern USA to sequester C. Our objectives were to compare tillage methods for soil CO2 flux, and determine if chemical or physical properties after 25 years of conventional or conservation tillage correlated with flux rates. Data were collected for several weeks during June and July in 2003, October and November in 2003, and April to July in 2004 from a tillage study established in 1978 on a Norfolk loamy sand (fine-loamy, kaolinitic, thermic Typic Kandiudults). Conventional tillage consisted of disking to a depth of approximately 15Â cm followed by smoothing with an S-tined harrow equipped with rolling baskets. Conservation tillage consisted of direct seeding into surface residues. Flux rates in conservation tillage averaged 0.84 g CO2 m-2 h-1 in Summer 2003, 0.36 g CO2 m-2 h-1 in Fall 2003, 0.46 g CO2 m-2 h-1 in Spring 2004, and 0.86 g CO2 m-2 h-1 in Summer 2004. Flux rates from conventional tillage were greater for most measurement times. Conversely, water content of the surface soil layer (6.5 cm) was almost always higher with conservation tillage. Soil CO2 flux was highly correlated with soil water content only in conventional tillage. In conservation tillage, no significant correlations occurred between soil CO2 flux and soil N, C, C:N ratio, pH, bulk density, sand fraction, or clay fraction of the surface 7.5 cm. In conventional tillage, sand fraction was positively correlated, while bulk density and clay fraction were negatively correlated with soil CO2 flux rate, but only when the soil was moist. Long-term conservation tillage management resulted in more uniform within- and across-season soil CO2 flux rates that were less affected by precipitation events.