Although agricultural soil management is the predominant anthropogenic source of nitrous oxide (N2O) to the atmosphere, little is known about the effects of alternative soil management practices on N2O emissions. In this study the NGAS model of Parton et al. (1996), coupled with a N and C cycling model, was used to simulate annual N2O emissions from 2639 cropland sites in the US using both no-till and conventional tillage management scenarios. The N2O mitigation potential of returning marginal cropland to perennial grass was also evaluated by comparing simulated N2O emissions from 306 Conservation Reserve Program (CRP) grassland sites with emissions from nearby cropland sites. Extensive soil and land use data for each site was obtained from the Natural Resource Inventory (NRI) database and weather data was obtained from NASA. The initial conversion of agricultural land to no-till showed greater N2O emissions per hectare than conventional tillage. Differences between the two tillage scenarios were strongly regional and suggest that conversion of conventionally tilled soil to no-till may have a greater effect on N2O emissions in drier regions. About 80% of the total emissions were from the Great plains and central regions mainly due to their large cultivated area. Croplands producing soy, wheat, and corn were responsible for about 68% of the total emissions with rice, cotton, and vegetable croplands having the greatest N2O flux (6.5-8.4 kg N2O-N ha-1 yr-1) under either scenario. Model simulations estimate that the agricultural lands in the US produce 448 Gg N2O-N y-1 under a conventional tillage scenario and 478 Gg N2O-N yr-1 under a no-till scenario. Model estimates also suggest that the conversion of 10.5 million hectares of cropland to grassland has a N2O mitigation potential of 31 Gg N2O-N yr-1, (8.4 Tg carbon equivalents yr-1). This value is similar in magnitude to many of the major greenhouse gas (GHG) emission-reduction strategies currently being considered to help meet US GHG reduction goals. Thus the GHG mitigation potential of this conversion is substantial and may be a viable strategy to help meet GHG reduction goals.