Although the Midwestern United States is one of the world's major agricultural production areas, few studies have assessed the effects of the region's predominant tillage and rotation practices on greenhouse gas emissions from the soil surface. Our objectives were to (a) assess short-term chisel (CP) and moldboard plow (MP) effects on soil CO2 and CH4 fluxes relative to no-till (NT) and, (b) determine how tillage and rotation interactions affect seasonal gas emissions in continuous corn and corn-soybean rotations on a poorly drained Chalmers silty clay loam (Typic Endoaquoll) in Indiana.