The Conservation Reserve Program (CRP) was initiated to reduce water and wind erosion on marginal, highly erodible croplands by removing them from production and planting permanent, soil-conserving vegetation such as grass. We conducted a field study at two sites in Wyoming, USA, in order to quantify changes in soil C and N of marginal croplands seeded to grass, and of native rangeland plowed and cropped to wheat-fallow. Field plots were established on a sandy loam site and a clay loam site on wheat-fallow cropland that had been in production for 60+ years and on adjacent native rangeland. In 1993, 6 years after the study was initiated, the surface soil was sampled in 2.5 cm depth increments, while the subsurface soil was composited as one depth increment. All soil samples were analyzed for total organic C and N, and potential net mineralized C and N. After 60+ years of cultivation, surface soils at both study sites were 18-26% lower (by mass) in total organic C and N than in the A horizons of adjacent native range. Six years after plowing and converting native rangeland to cropland (three wheat-fallow cycles), both total and potential net mineralized C and N in the surface soil had decreased and NO3-N at all depths had increased to levels found after 60+ years of cultivation. We estimate that mixing of the surface and subsurface soil with tillage accounted for 40-60% of the decrease in surface soil C and N in long-term cultivated fields; in the short-term cultivated fields, mixing with tillage may have accounted for 60-75% of the decrease in C, and 30-60% of the decrease in N. These results emphasize the need to evaluate C and N in the entire soil solum, rather than in just the surface soil, if actual losses of C and N due to cultivation are to be distinguished from vertical redistribution. Five years after reestablishing grass on the sandy loam soil, both total and potential net mineralized C and N in the surface soil had increased to levels equal to or greater than those observed in the A horizon of the native range. On the clay loam soil, however, significant increases in total organic C were observed only in the surface 2.5 cm of N-fertilized grass plots, while total organic N had not significantly increased from levels observed in the long-term cultivated fields.
